Microtubule-dependent ribosome localization in C. elegans neurons.

Subcellular localization of ribosomes defines the location and capacity for protein synthesis. Methods for in vivo visualizing ribosomes in multicellular organisms are desirable in mechanistic investigations of the cell biology of ribosome dynamics. Here, we developed an approach using split GFP for tissue-specific visualization of ribosomes in Caenorhabditis elegans. Labeled ribosomes are detected as fluorescent puncta in the axons and synaptic terminals of specific neuron types, correlating with ribosome distribution at the ultrastructural level. We found that axonal ribosomes change localization during neuronal development and after axonal injury. By examining mutants affecting axonal trafficking and performing a forward genetic screen, we showed that the microtubule cytoskeleton and the JIP3 protein UNC-16 exert distinct effects on localization of axonal and somatic ribosomes. Our data demonstrate the utility of tissue-specific visualization of ribosomes in vivo, and provide insight into the mechanisms of active regulation of ribosome localization in neurons

Visual function and serous retinal detachment in patients with branch retinal vein occlusion and macular edema: a case series

<p>Abstract</p> <p>Background</p> <p>The influence of serous retinal detachment (SRD) on retinal sensitivity in patients with branch retinal vein occlusion (BRVO) and macular edema remains unclear. This is despite the frequent co-existence of SRD and cystoid macular edema (CME) in BRVO patients on optical coherence tomography (OCT) and the fact that CME is the most common form of macular edema secondary to BRVO. We investigated visual function (visual acuity and macular sensitivity), macular thickness, and macular volume in patients with BRVO and macular edema.</p> <p>Methods</p> <p>Fifty-three consecutive BRVO patients (26 women and 27 men) were divided into two groups based on optical coherence tomography findings. Macular function was documented by microperimetry, while macular thickness and volume were measured by OCT.</p> <p>Results</p> <p>There were 15 patients with SRD and 38 patients with CME. Fourteen of the 15 patients with SRD also had CME. Visual acuity was significantly worse in the SRD group than in the CME group (P = 0.049). Also, macular thickness and macular volume within the central 4°, 10°, and 20° fields were significantly greater in the SRD group (P = 0.008, and P = 0.007, P < 0.001 and P < 0.001, and P < 0.001 and P < 0.001, respectively). However, macular sensitivity within the central 4°, 10°, and 20° fields was not significantly worse in the SRD group than in the CME group.</p> <p>Conclusions</p> <p>SRD itself may decrease visual acuity together with CME, because nearly all SRD patients also had CME. SRD does not seem to influence macular function on microperimetry.</p

Fertility Status of Fadama Soils in Gantsare Village, Wamakko Local Government, Sokoto State As Affected by Cement Dust

A study was conducted in Gantsare village, Wamakko local government, Sokoto state to determine the effect of cement dust emitted from Sokoto cement factory on the fertility of fadama soils. Soil samples were collected in June, 2008 from the eastern, northeastern, and southeastern parts of the village and Girabshi; a nearby village with least effect of the dust. The samples were collected at two depths (0-15 and 15-30 cm) using simple random sampling method. Randomized complete block design (RCBD) was employed, replicated three times, making a total of 24 samples. The samples were analyzed for pH, OC, CEC, total N, available P, exchangeable bases (Ca, Mg, K and Na) and particle size. Almost all parameters determined were found to be statistically significant (P < 0.01). OC was found to be lower in concentration in the areas closer to the factory; eastern part6.25 respectively, likewise CEC and exchangeable bases. The trend in the results observed may be attributed to possible adverse effects of the dust on microbial population, reduction in the availability of some nutrients and a favour on the concentrations of exchangeable bases and CEC. The topsoils showed better results of all the soil chemical properties. The study recommends minimization of the amount of dust reaching agricultural soils and the use of phosphorus and nitrogen fertilizer supplements as soil management practices to enhance soil fertility status and enhance adequate crop yield in the area. Key words:Cement dust, fertility status, fadama soils, soil chemical properties, soil management

Visual acuity and foveal thickness after vitrectomy for macular edema associated with branch retinal vein occlusion: a case series

Abstract Background The mechanism by which vitrectomy improves macular edema in patients with branch retinal vein occlusion remains unclear, although intraocular levels of vascular endothelial growth factor have been suggested to influence the visual prognosis and macular edema. Methods A series of 54 consecutive patients (54 eyes) with branch retinal vein occlusion was studied prospectively. All patients underwent pars plana vitrectomy for treatment of macular edema. Best corrected visual acuity and retinal thickness (examined by optical coherence tomography) were assessed before and after surgery. The level of vascular endothelial growth factor in vitreous fluid harvested at operation was determined. Patients were followed for at least 6 months postoperatively. Results Both the visual acuity and the retinal thickness showed significant improvement at 6 months postoperatively (P = 0.0002 and P Conclusions These results suggest that the vitreous level of vascular endothelial growth factor might influence the visual prognosis and the response of macular edema to vitrectomy in patients with branch retinal vein occlusion.</p

Ipomea asarifolia (Desr), A Potential Cover Crop for Soil Fertility Improvement in The Sudan Savanna Region, Nigeria

The effect of Ipomea asarifolia on physico - chemical properties of the soils at Usmanu Danfodiyo University, Sokoto main campus, in the Sudan Savanna of Nigeria was investigated. The study determined the effect of the plant on some physical and chemical properties of soils, found out the nutrient retention ability of the plant in the soils and the variation of the effect with distance within the plants’ canopy. A total of 48 samples were collected from four different sites within Usmanu Danfodiyo University main Campus; VC complex area, University stadium area, Behind new library area and opposite IBB centre area and four distances from the plant (control, plant base, 0.5 m and 1 m from the plant base). Samples were collected for use in a randomized complete block design (RCBD) experiment with three replicates. Soil pH, Organic C, CEC, total N, available P, K, Ca and Mg concentrations were determined, particle size analysis was also carried out. The results were subjected to analysis of variance (ANOVA), where significant F values were obtained, the means were separated by Duncan multiple range tests (DMRT). The difference among the sites and the distances were found to be statistically significant (P VC complex area > New library area > IBB centre area. On the other hand, the effect on all the parameters was found to decrease with distance from the plant with the base of the plant having the highest organic C, CEC, total N, available P and exchangeable K, Ca and Mg; 6.83 g kg-1, 5.00 cmol kg-1, 0.42 g kg-1, 10.82 mg kg-1 and 0.26, 0.50 and 0.97 cmol kg-1 respectively, while the control soils had the least values; 3.46 g kg-1, 4.00 cmol kg-1, 0.12 g kg-1, 7.43 mg kg-1, 0.21, 0.38, and 0.45 cmol kg-1 respectively. The effect was attributed to the accumulation of organic matter around the plant and its ability to prevent erosion by both water and wind thus retaining nutrients making it a suitable cover crop for soil fertility improvement

What does touch tell us about emotions in touchscreen-based gameplay?

This is the post-print version of the Article. The official published version can be accessed from the link below - Copyright @ 2012 ACM. It is posted here by permission of ACM for your personal use. Not for redistribution.Nowadays, more and more people play games on touch-screen mobile phones. This phenomenon raises a very interesting question: does touch behaviour reflect the player’s emotional state? If possible, this would not only be a valuable evaluation indicator for game designers, but also for real-time personalization of the game experience. Psychology studies on acted touch behaviour show the existence of discriminative affective profiles. In this paper, finger-stroke features during gameplay on an iPod were extracted and their discriminative power analysed. Based on touch-behaviour, machine learning algorithms were used to build systems for automatically discriminating between four emotional states (Excited, Relaxed, Frustrated, Bored), two levels of arousal and two levels of valence. The results were very interesting reaching between 69% and 77% of correct discrimination between the four emotional states. Higher results (~89%) were obtained for discriminating between two levels of arousal and two levels of valence

The geographic distribution of onchocerciasis in the 20 participating countries of the African Programme for Onchocerciasis Control:(2) pre-control endemicity levels and estimated number infected

BACKGROUND: The original aim of the African Programme for Onchocerciasis Control (APOC) was to control onchocerciasis as a public health problem in 20 African countries. In order to identify all high risk areas where ivermectin treatment was needed to achieve control, APOC used Rapid Epidemiological Mapping of Onchocerciasis (REMO). REMO involved spatial sampling of villages to be surveyed, and examination of 30 to 50 adults per village for palpable onchocercal nodules. REMO has now been virtually completed and we report the results in two articles. A companion article reports the delineation of high risk areas based on expert analysis. The present article reports the results of a geostatistical analysis of the REMO data to map endemicity levels and estimate the number infected. METHODS: A model-based geostatistical analysis of the REMO data was undertaken to generate high-resolution maps of the predicted prevalence of nodules and of the probability that the true nodule prevalence exceeds the high risk threshold of 20%. The number infected was estimated by converting nodule prevalence to microfilaria prevalence, and multiplying the predicted prevalence for each location with local data on population density. The geostatistical analysis included the nodule palpation data for 14,473 surveyed villages. RESULTS: The generated map of onchocerciasis endemicity levels, as reflected in the prevalence of nodules, is a significant advance with many new endemic areas identified. The prevalence of nodules was > 20% over an area of 2.5 million km2 with an estimated population of 62 million people. The results were consistent with the delineation of high risk areas of the expert analysis except for borderline areas where the prevalence fluctuated around 20%. It is estimated that 36 million people would have been infected in the APOC countries by 2011 if there had been no ivermectin treatment. CONCLUSIONS: The map of onchocerciasis endemicity levels has proven very valuable for onchocerciasis control in the APOC countries. Following the recent shift to onchocerciasis elimination, the map continues to play an important role in planning treatment, evaluating impact and predicting treatment end dates in relation to local endemicity levels

Integrable Structure of 5d5d N=1\mathcal{N}=1 Supersymmetric Yang-Mills and Melting Crystal

We study loop operators of $5d$ $\mathcal{N}=1$ SYM in $\Omega$ background. For the case of U(1) theory, the generating function of correlation functions of the loop operators reproduces the partition function of melting crystal model with external potential. We argue the common integrable structure of $5d$ $\mathcal{N}=1$ SYM and melting crystal model.Comment: 12 pages, 1 figure, based on an invited talk presented at the international workshop "Progress of String Theory and Quantum Field Theory" (Osaka City University, December 7-10, 2007), to be published in the proceeding

A new solution to the problem of finding all numerical solutions to ordered metric structures

A new algorithm is used to test and describe the set of all possible solutions for any linear model of an empirical ordering derived from techniques such as additive conjoint measurement, unfolding theory, general Fechnerian scaling and ordinal multiple regression. The algorithm is computationally faster and numerically superior to previous algorithms.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/45740/1/11336_2005_Article_BF02293603.pd

Unusual orbital lymphoma undetectable by magnetic resonance imaging: a case report

<p>Abstract</p> <p>Introduction</p> <p>We report the case of a patient with orbital malignant lymphoma that was not detected by imaging studies when she presented with impaired vision, which lead to her eventual loss of sight.</p> <p>Case presentation</p> <p>A 71-year-old Japanese woman complained of deteriorating vision in her left eye. On examination, papilledema was detected, but magnetic resonance imaging only showed slight thickening and enhancement of the left optic nerve. A diagnosis of idiopathic optic neuritis was made and corticosteroid pulse therapy was administered. During the next four months, the patient received a total of four courses of corticosteroid pulse therapy, but she still suffered from bilateral loss of vision. A second magnetic resonance imaging procedure revealed tumors in both orbits and a biopsy showed diffuse large B-cell malignant lymphoma.</p> <p>Conclusion</p> <p>The possibility of malignant lymphoma should be considered in patients with recurrent optic neuropathy despite administration of corticosteroid pulse therapy, even when there are no abnormalities on cerebrospinal fluid examination or magnetic resonance imaging.</p